Figure 6
Comparison of NCp7 and NCp8 DNA strand exchange activity. (A) Schematic of the DNA/DNA strand exchange reaction. (B) A representative electrophoretic analysis of DNA strand exchange in the R1(+)DNA/R1(−) DNAmut duplex at increasing concentrations of NCp7 or NCp8. Lane 1: R1(+) DNA only; lane 2: heat-annealed R1(+) DNA/R1(−)DNA; lane 3: heat-annealed R1(+) DNA/R1(−) DNAmut; lane 4: the heat-annealed R1(+)DNA/R1(−) DNAmut duplex incubated with R1(−) DNA; lanes 5 – 9: strand exchange reaction at 0.06, 0.125, 0.25, 0.5 and 1 μM NCp7; lanes 10 – 14: strand exchange reaction at 0.06, 0.125, 0.25, 0.5 and 1 μM NCp8. (C) Percentage of R1(−) DNAmut exchanged, measured as a ratio of perfect to imperfect duplex. (D) Percentage of TAR1(−) DNAmut exchanged, measured as a ratio of perfect to imperfect duplex. The graphs represent the averaged data from three independent experiments. The error bars represent standard deviations.