Comparison of NCp7, NCp8 or (8–48) NCp8 annealing activity. The assays were performed using different DNA and RNA substrates as a function of protein concentration as indicated. Annealing of TAR1(−)DNA/TAR1(+)DNA (A), TAR1(−)DNA/TAR1 RNA (B), R1(−)DNA/R1(+)DNA (C), TAR2(−)DNA/TAR2 RNA (D). The graphs represent averaged data from three independent gel shift annealing experiments for each protein. The error bars represent standard deviations.