Figure 5

Active ADAM17 associates with exosomes from F12/Hut-78 cells. Western blot analysis for the expression of ADAM17 in both cells (A) and exosomes (B) from Hut-78 and F12/Hut-78 cells. On the left of blots for ADAM17, arrows identify both inactive and active ADAM17 forms. Signals from cellular ADAM17 were normalized with β-actin signals, while both anti-ICAM-1 and anti-CD63 monoclonal antibodies served to normalize ADAM17 signals from exosomes. On the right of each panel, molecular weight markers are given in kDa. Results are representative of five independent experiments.