Figure 3
OMM localization of HIV-1 PR and BCA3. Purified mitochondria from HEK-293 cells co-transfected with c-myc-HIV-1 PR(D25N) and HA-BCA3 were incubated with 0.14 μM (a), 1.4 μM (b) or 14 μM (c) proteinase K in the presence or absence of Triton X-100 for 10 and 60 min on ice. The reaction was stopped by the addition of 6× protein loading buffer (PLB), and samples were analyzed by Western blotting.