infection of human microglia induces rapid ASC intracellular translocation. A. Mock-infected microglia exhibit minimal ASC immunoreactivity. B. At 1 hr post-infection ASC immunoreactivity (red) was detected in the cytoplasm. C. At 2 hr post-infection, punctate ASC immunoreactivity was evident in the cytoplasm of microglia. D. At 3 hr post-infection, aggregates of ASC immunoreactivity was readily apparent in microglia. (Original magnification 400X).