Skip to main content
Figure 4 | Retrovirology

Figure 4

From: Rapid inflammasome activation in microglia contributes to brain disease in HIV/AIDS

Figure 4

IL-1β release in response to HIV-1 SF162 exposure is dependent on the NLRP3 inflammasome but not viral fusion or CCR5. A. Induction, processing and B. release of IL-1β from PMA-differentiated THP-1 cells following 4 hr exposure to HIV-1SF162. C. IL-1β release from PMA-differentiated THP-1 cells exposed to HIV-1SF162 for 4 hr +/- extracellular KCl (50 mM) or YVAD-fmk (80 μM). D. Release of IL-1β from PMA-differentiated THP-1 cells following 18 hr exposure to HIV-1SF162 +/- the inhibitor of viral fusion T20 (20 μg/mL) or the CCR5 antagonist maraviroc (100nM). E. IL-1β release from PMA-differentiated THP-1 cells or THP1-defNLRP3 cells exposed to HIV-1SF162 or poly dA:dT for 4 hr (poly dA:dT was transfected using Lipofectamine 2000). F. IL-1β release from PMA-differentiated THP-1 cells exposed to HIV-1YU2, HxBRuR+E- + VSVG, or HxBRuR+E- + HIV-1Env3098 for 24 hours. Mean levels are reported for each ELISA. Bars indicate standard error. (*p < 0.05). “N.D.” indicates that IL-1β was “Not Detected”.

Back to article page