Stable 293 T and CHO cell lines expressing cleaved, stable HIV-1 envelope glycoprotein trimers for structural and vaccine studies

Table 1 Production of BG505 SOSIP.664 gp140 trimers by stable cell lines relative to transiently transfected cells

Production system	Purified trimer yield per 1 × 10⁹cells
CHO PEI transfection	0.25-0.5 mg
CHO stable cell line	10-15 mg
293 T PEI transfection	1.25-1.5 mg
293 T stable cell line	10-15 mg

ISSN: 1742-4690