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Table 9 The VRC01 susceptibility of AE-Env clones containing D185 and the derived mutants

From: Impact of amino acid substitutions in the V2 and C2 regions of human immunodeficiency virus type 1 CRF01_AE envelope glycoprotein gp120 on viral neutralization susceptibility to broadly neutralizing antibodies specific for the CD4 binding site

 

IC50of VRC01 (μg/ml)a

 

Mutation(s)b

Env clone

Wild-type

N186Q

N197Q

D185G/N186Q

D185G/N197Q

29CC1

>2 c

 

>2

 

>2

45CC1

>2

 

0.05

 

0.09

47PL1

>2

 

>2

 

>2

99PB2

0.02

 

0.10

 

0.03

99CC8

1.16

 

0.20

 

0.06

105PB1

0.07

 

0.11

 

0.04

105PL2

>2

 

0.07

 

0.09

105PL3

0.25

 

0.02

 

0.02

107CC2

0.33

0.15

 

0.09

 
  1. aIC50 of VRC01 for suppressing viral replication was calculated using GraphPad Prism 5 software. Data are shown as the means of at least two independent experiments.
  2. bSingle or multiple amino acid mutations were introduced into the AE Env-recombinant virus. Amino acid numbering is based on HXB2 Env gp120.
  3. cIC50 is >2 μg/ml.