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Table 5 The b12 susceptibility of AE-Env clones containing D185 and the derived mutants

From: Impact of amino acid substitutions in the V2 and C2 regions of human immunodeficiency virus type 1 CRF01_AE envelope glycoprotein gp120 on viral neutralization susceptibility to broadly neutralizing antibodies specific for the CD4 binding site

 

IC50of b12 (μg/ml)a

 

Mutation(s)b

Env clone

Wild-type

N186Q

N197Q

N186Q/N197Q

D185G/N186Q

D185G/N197Q

D185N/N197Q

D185E/N197Q

D185G/N186Q/N197Q

29CC1

>40c

>40

0.10

8.34

 

>40

12.59

>40

>40

45CC1

>40

>40

0.02

0.65

 

>40

5.77

0.45

>40

47PL1

>40

 

0.03

  

>40

>40

5.30

 

99PB2

>40

 

3.65

  

>40

   

99CC8

>40

 

0.10

  

>40

   

105PB1

>40

>40

0.07

0.16

 

>40

7.03

>40

>40

105PL2

>40

 

0.29

  

>40

   

105PL3

>40

>40

0.03

0.24

 

>40

10.09

>40

>40

107CC2

>40

0.02

  

3.42

    
  1. aIC50 of b12 for suppressing viral replication was calculated using GraphPad Prism 5 software. Data are shown as the means of at least three independent experiments.
  2. bSingle or multiple amino acid mutations were introduced into the AE Env-recombinant virus. Amino acid numbering is based on HXB2 Env gp120.
  3. cIC50 is >40 μg/ml.
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Retrovirology

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