Impact of amino acid substitutions in the V2 and C2 regions of human immunodeficiency virus type 1 CRF01_AE envelope glycoprotein gp120 on viral neutralization susceptibility to broadly neutralizing antibodies specific for the CD4 binding site

Table 3 Amino acid residue at position 185 of HIV-1 Env

	n	D185^a	E185^a	G185^a	N185^a	Others^a
Subtype B^b	200	70.5%	6.5%	4.5%	7.5%	11%
Subtype C^b	200	19%	15%	7.5%	36%	22.5%
CRF01_AE^b	200	24%	27.5%	22%	16%	10.5%
b12 susceptible CRF01_AE^c	15	66.7%	13.3%	6.7%	6.7%	6.7%
b12 resistant CRF01_AE^d	17	11.8%	17.6%	17.6%	29.4%	23.5%

^aFrequency (%) of samples with aspartic acid residue (D185), glutamic acid residue (E185), glycine residue (G185), asparagine residue (N185) or other residues, alanine, histidine, lysine, methionine, proline, glutamine, arginine, serine, threonine, valine or a gap (others) at amino acid position 185 of Env was estimated.
^b200 env gene sequences of subtype B, C or CRF01_AE viruses with sampling dates 2008–2010, 2008–2009 or 2007–2010, respectively, were retrieved from the HIV sequence database (http://www.hiv.lanl.gov). The deduced amino acid sequences were translated and examined. Accession numbers are available upon request.
^cAE-Env clones which became b12 susceptible after removing N186 and/or N197.
^dAE-Env clones which were b12 resistant after removing N186 and/or N197.

ISSN: 1742-4690