Skip to main content
Figure 5 | Retrovirology

Figure 5

From: Impact of amino acid substitutions in the V2 and C2 regions of human immunodeficiency virus type 1 CRF01_AE envelope glycoprotein gp120 on viral neutralization susceptibility to broadly neutralizing antibodies specific for the CD4 binding site

Figure 5

The binding efficiency of b12 to Env proteins on viral particles. Recombinant viruses containing wild-type AE-Env clones, 47CC11, 50PB2 and 52PB3, a wild-type B-Env clone, pNL4-3, and AE-Env mutants, 47CC11-G185D, 47CC11-N197Q, 47CC11-G185D/N197Q, 50PB2-N197Q, 50PB2-N185D/N197Q, 52PB3-N197Q and 52PB3-E185D/N197Q, were subjected to the study. The binding efficiency of b12 to viral particle-associated Env proteins was evaluated as described in Methods. Data are shown as the percent recovery of Gag p24 antigen, which was associated with virus-b12-magnetic bead complex relative to the input amount in an assay. The results are presented as the means and standard errors (error bars) of three independent experiments. Differences among the binding efficiencies of b12 to the wild-type and mutant AE-Env proteins were analyzed by the paired t test and are reported when P <0.05.

Back to article page