Figure 6

The two proviral copies are independently and differentially bound by the silencing complex. (A) ChIP–based measurement of H3K9me3 mark, Trim28 and YY1 present on the proviral DNAs in sorted populations. ChIP data is determined as recovered DNA relative to input DNA, and the ratio between occupancy on the GFP gene to the occupancy on the average of both proviruses in the U5-PBS region is presented. The values are finally presented as compared with the values for the double-negative sorted population, with the double-negative cells normalized to 1. (B) Same as A, ratio between occupancy on mCherry to that on the total U5-PBS region is presented, again with the ratio for the double-negative sorted cells normalized to 1.