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Figure 7 | Retrovirology

Figure 7

From: Nuclear import of SAMHD1 is mediated by a classical karyopherin α/β1 dependent pathway and confers sensitivity to VpxMAC induced ubiquitination and proteasomal degradation

Figure 7

SAMHD1 K11A is ubiquitinated in the presence of Vpx MAC . A) 293 T cells were co-transfected with plasmids encoding HA-tagged wild type or K11A mutant SAMHD1 along with plasmids expressing VpxMAC or VpxRCM and plasmids encoding either HIS-tagged wild type ubiquitin (HIS-Ubi) or K48R mutant ubiquitin (HIS-UbiK48R) for 24 h before cells were lysed and subjected to NiNTA pull down. Cell lysates (Input) or pulled down samples (NiNTA PD) were subjected to SDS-PAGE using 10% gels and western blotting using an anti-HA antibody. Mono- and polyubiquitinated SAMHD1 are indicated. B) Similar experiment as shown in A) but using ubiquitin mutants HIS-UbiK6R, K11R, K27R, K29R, K33R, K48R or K63R.

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Retrovirology

ISSN: 1742-4690

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