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Figure 3 | Retrovirology

Figure 3

From: Nuclear import of SAMHD1 is mediated by a classical karyopherin α/β1 dependent pathway and confers sensitivity to VpxMAC induced ubiquitination and proteasomal degradation

Figure 3

SAMHD1 WT and SAMHD1 K11A bind Vpx MAC with similar efficiencies. 293 T cells were co-transfected with plasmids expressing GST, GST-SAMHD1 or GST-SAMHD1K11A along with plasmids expressing Flag-tagged Vpx derived from HIV-2ROD, SIVMAC or SIVRCM. GST-pull down was performed and samples were separated using 15% SDS-PAGE gels. Vpx proteins were detected using an anti-Flag antibody, and SAMHD1 was detected using an anti-GST antibody. Tubulin served as a loading control for the input.

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Retrovirology

ISSN: 1742-4690

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