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Figure 3 | Retrovirology

Figure 3

From: Diverse viral glycoproteins as well as CD4 co-package into the same human immunodeficiency virus (HIV-1) particles

Figure 3

Co-capture of HIV-1 Env and MLV Env. A & B) Co-capture was performed using virus with HIV-1 Env and MLV Env. Luciferase signal from A) 293 T mCAT-1 cells or B) TZM-bl cells infected with virus produced in the presence of MLV Env, HIV-1 Env or both Envs and then subjected to the following: anti-MLV Env capture + wash, anti-HIV capture + wash, no antibody capture + wash & straight infection (no antibody, no wash). The signal was normalized to the straight infection positive control from the relevant single glycoprotein virus sample. All graphs are the average of at least three independent experiments unless indicated otherwise and show standard deviation. C & D) Luciferase signal as before from C) 293 T mCAT-1 and D) TZM-bl cells infected with virus produced with only MLV Env, only HIV Env or a mixture of the two viral supernatants. A & B were the average of at least 3 independent experiments, C & D were the average of two experiments.

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