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Figure 4 | Retrovirology

Figure 4

From: Interferon-induced HERC5 is evolving under positive selection and inhibits HIV-1 particle production by a novel mechanism targeting Rev/RRE-dependent RNA nuclear export

Figure 4

HERC5 targets Rev/RRE function. A, Schematic depicting the different Gag constructs used in the experiment. 293T cells were co-transfected with increasing concentrations of plasmids encoding HERC5 and either Rev-dependent GagPol (containing RRE) +/- Rev (B), Rev-independent GagPol (containing 4xCTE in place of the RRE) (C), or Rev-independent Gag-only (codon-optimized) construct (D). Total DNA transfected was kept equal with empty vector plasmid. Gag levels within the cell lysates were analyzed by quantitative Western blotting using anti-p24CA and anti-β-actin as a loading control. Numbers below the bands represent densitometric quantification of the indicated bands relative to the empty vector control after normalization to β-actin levels.

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Retrovirology

ISSN: 1742-4690

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