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Figure 2 | Retrovirology

Figure 2

From: Interferon-induced HERC5 is evolving under positive selection and inhibits HIV-1 particle production by a novel mechanism targeting Rev/RRE-dependent RNA nuclear export

Figure 2

Endogenous HERC5 inhibits intracellular Gag protein production. A, 293T cells were co-transfected with pR9 and either pScram or pHERC5shRNA. Forty-eight hours after transfection, cell lysates were subjected to Western blotting using anti-p24CA or anti-β-actin as a loading control. B, Cells were transfected as in part A for 24 hours and then treated with 500 units/ml of IFN-β. Sixteen hours after IFN-β treatment, cell lysates were subjected to Western blotting using anti-p24CA or anti-β-actin as a loading control. Data shown in panels A and B are representative of at least three independent experiments. C, Human monocyte-derived macrophages from two different donors were transfected as in panel A. Seventy-two hours after transfection, cell lysates were subjected to Western blotting using anti-p24CA or anti-β-actin as a loading control.

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Retrovirology

ISSN: 1742-4690

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