Reverse transcription is additionally impaired in clinically derived transmitted/founder viruses. MDM were from the same donor infected with equal amounts of p24 YU-2 and CH077 viruses. (A) MDM were fixed and stained for intracellular p24 protein 48 h post-infection. (B,C) MDM were harvested and total DNA was isolated at 0 h, 6 h and 18 h post-infection. Viral DNA products were detected using quantitative PCR. (B) total DNA; (C) strong stop. This is a representative example of three independent experiments on different donors. The YU-2:CH077 ratio represents fold difference in infection or viral DNA copies numbers between these viruses.