Figure 5
From: A novel HIV-1-encoded microRNA enhances its viral replication by targeting the TATA box region
MiR-H3 targets HIV-1 5′ LTR and upregulates HIV-1 promoter activity. (A) Effects of miR-H3 overexpression on different HIV-1 derived lentiviral vectors. pNL4-3-deltaE-EGFP, pCMV-ΔR8.2 and pAX2 are all HIV-1 derived lentiviral vectors with major difference in their 5′ and 3′ ends: pNL4-3-deltaE-EGFP contains HIV-1 LTRs, the other two contain most viral protein genes which were driven by a CMV promoter. MiR-H3 precursor was co-transfected with these vectors separately, and then the HIV-1 total transcripts from these plasmids were determined by real-time qRT-PCR. (B) Dual-Luciferase assay was performed on the reporter construct with a HIV-1 5′-LTR functioning as the promoter or a HIV-1 3′-LTR sequence as the 3′-UTR region after co-transfection with the miR-H3 precursor or the empty vector. P-values were calculated using the two tailed unpaired Student’s t-test with equal variances, n = 3. *p < 0.05, **p < 0.01.