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Figure 4 | Retrovirology

Figure 4

From: A novel HIV-1-encoded microRNA enhances its viral replication by targeting the TATA box region

Figure 4

MiR-H3 upregulates HIV-1 total RNA and protein level. (A) Real-time qRT-PCR analysis of HIV-1 total transcripts from HEK293T cells transfected with pNL4-3-deltaE-EGFP and a construct harboring the miR-H3 precursor or the empty vector. (B) pNL4-3-deltaE-EGFP or a miR-H3-defective construct pNL4-3-deltaE-EGFP-miRH3-mut was transfected into HEK293T cells and total HIV-1 transcripts were assayed with real-time qRT-PCR at 48 hrs post-transfection. GAPDH was set as the internal control. (C) Western blot of HIV-1 structural protein P55, P41 and P24 in HEK293T cells transfected with pNL4-3-deltaE-EGFP and the empty vector, the construct containing miR-150 precursor or miR-H3 precursor. The β-actin was used as an internal control. P-values were calculated using the two tailed unpaired Student’s t-test with equal variances, n = 3. *p < 0.05, ***p < 0.001.

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