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Figure 1 | Retrovirology

Figure 1

From: Human protein Staufen-2 promotes HIV-1 proliferation by positively regulating RNA export activity of viral protein Rev

Figure 1

Rev interacts with hStau-2. A) Co-immunoprecipitation of endogenous hStau-2 with Rev-GFP using anti-Rev antibody and Western Blot with anti-hStau-2 antibody: HEK293T cells were transfected with Rev-EGFP vector or GFP vector control, cell lysates were prepared after 48 hours followed by IP and WB. Whole cell lysates were checked for Rev expression by anti-Rev antibody. B) Co-immunoprecipitation of endogenous hStau-2 with Rev-GFP using anti-GFP antibody and Western Blot with anti-hStau-2 antibody: HEK293T cells were transfected with Rev-EGFP vector or GFP vector control, cell lysates were prepared after 48 hours followed by IP and WB. Whole cell lysates were checked for GFP/Rev-GFP expression by anti-GFP antibody. C) RNase A treatment and pulldowns: Cell lysates were treated with RNase A. RNA degradation was assessed by RNA isolation from both RNase A untreated and treated cell lysates and run on 2% Agarose gel (1st Panel). Treated and untreated samples were used for IP with anti-Rev antibody and WB by anti-Staufen-2 antibody (2nd Panel) and anti-Rev antibody (3rd Panel). D) RNase III treatment and pulldowns: Cell lysates were treated with RNase III. RNA degradation was assessed by RNA isolation from both RNase III untreated and treated cell lysates and run on 2% Agarose gel (1st Panel). Treated and untreated samples were used for IP with anti-Rev antibody and WB by anti-Staufen-2 antibody (2nd Panel) and anti-Rev antibody (3rd Panel).

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