Figure 7

The productivity of RGH infection is determined within four days post-infection. A: Jurkat cells were infected with RGH. Four days post-infection, cells were treated with either DMSO or TNFα for 24 hours before analysis by flow cytometry. Cells were left to recover for four days prior to re-analysis by flow cytometry. The percentage of cells in each of the eGFP- mCherry-, eGFP- mCherry+, and eGFP+ mCherry+ quadrants is shown in the circle plots. Data shown is representative of duplicate experiments. B: Jurkat cells were infected with RGH and sorted three days post-infection into eGFP- mCherry-, eGFP- mCherry+, and eGFP+ mCherry+ (>90% purity). After 24 hours, the sorted cells were treated with either DMSO or TNFα for 24 hours prior to analysis by flow cytometry. Treated cells were left to recover for four days prior to further flow cytometry analysis. Data are representative of duplicate experiments.