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Table 4 Oligonucleotides used for NL4.3 GFP E- quantitative PCR (De Iaco, 2012 and Butler, 2001)

From: Vpx rescue of HIV-1 from the antiviral state in mature dendritic cells is independent of the intracellular deoxynucleotide concentration

  Primer name Primer sequence
Late RT J1 fwd 5′-ACAAGCTAGTACCAGTTGAGCCAGATAAG-3′
J2 rev 5′- GCCGTGCGCGCTTCAGCAAGC-3′
LRT-P 5′-(FAM)-CAGTGGCGCCCGAACAGGGA-(TAMRA)-3′
2-LTR MH535 5′-AACTAGGGAACCCACTGCTTAAG-3′
MH536 5′-TCCACAGATCAAGGATATCTTGTC-3′
JunctPro 5′- (FAM)-CTCTAGCAGTACTGGAAGGGCTA-(TAMRA)-3′
Alu PCR MH535 5′-AACTAGGGAACCCACTGCTTAAG-3′
SB704 rev 5′-TGCTGGGATTACAGGCGTGAG-3′
MH603 probe 5′-(FAM)-ACACTACTTGAAGCACTCAAGGCAAGCTTT-(TAMRA)-3′
Mito. DNA MH533 5′-ACCCACTCCCTCTTAGCCAATATT-3′
MH534 5′-GTAGGGCTAGGCCCACCG-3′
Mito probe 5′-(TET)-CTAGTCTTTGCCGCCTGCGAAGCA-(TAMRA)-3′