Table 1 Codon optimized Vpx and Vpr sequences
Accessory gene | Codon-optimized nucleic acid sequence |
|---|---|
SIV MAC-251 Vpx | atgagcgacccaagagaaagaatcccacctggaaatagcggcgaagaaactattggagaggctttcgagtggctgaatagaaccgtggaggagataaatagagaagctgtgaaccatctgcccagagagctgatcttccaagtgtggcaaaggagctgggagtattggcacgacgagcagggcatgtcccagagctatgtgaaatatagatatctgtgtctgatgcagaaggcactgttcatgcactgtaaaaagggctgtaggtgcctcggggaaggacatggggccggcggatggaggcccggcccacctcctccccctccccccggcctcgcatga |
HIV-2 ROD Vpx | atgacagatccacgagagaccgtacccccaggcaacagtggagaagaaaccattggcgaggcgttcgcatggctcaacaggacggtggaggccatcaacagagaagccgtaaatcacctgcccagggaacttatctttcaggtctggcagaggagctggcggtactggcacgacgagcagggcatgtctgagagctataccaaataccgctacctttgtatcatccagaaggccgtttacatgcacgtgagaaaaggatgtacatgcttgggaagaggtcacggccctggcggctggagacctggcccaccaccccctcccccacctgggctggtgtga |
SIV RCM Vpx | atggctgagcgggcaccagaagtgccaactggcgccggcgaggccgagtttcagccctggctccgggacatgttggagaaagtcaacctggaggcccggttgcacttccaccccgaattcatctttaggctgtggagaacatgcgtcgagcactggcatgatgtgcaccagaggtccctggagtacgccgcctataggtacctcctcctgatgcagaaggccctgttcattcactgccagaccgggtgtagccaaagacatgggcccaatcctagggctgtgggagagcgcattacaatcctgcctgggatgtga |
SIV MUS1CM1239 Vpr | atggagagggtgcccccatcacatcggcccccatggcactccagggtggtcccaactaccatgcagcaggcacagcaggctatgtgggacctgaacgaggaagccgagaagcacttcagcagagaggagctgcggggaatctggaacgatgtcaccgagctccccgccgatcccaactggaccgtggatcaggccgctattgcctgtgccattgattacattcggcggactcagacactcctgtttcggcactacagggaaggctgctatcaccggtacagcaacacaatccgcaggtaccctaacatcagacccttgcgcgggacacaagcccctcccagtaacagcatgccaaatgccgaccctacacctccccttagaccctctaggtacaggatggacgagtga |
SIV DEBCM5 Vpr | atggagcgctatcctcctagtcatcccccacatttcacatccagaactgtcccaatgacccggctggcactgcagcaggccatgcaggacctgaacgaggaggccctgaagcacttcaccagggaagagttgtggggggtgtggaaccactgtgtcgatttgcccgcccagcccgattggacaggagagcaggcctgggccgctagcgtgatcgattacattaaaatcgtgcaaaggatgctctggctccaccttagggaggcttgctttcaccgggagagagaggccacacggcggtaccccaacattaggccactgaccggccggaatagggaggtgagagacggggaatga |