CypA protein level in different cell lines does not correlate with CsA-dependency. CypA protein levels by western blot in HeLa, AKATA (AKA), A549, TE671, Jurkat T (JUR), W132, BL41 and MT4 cell lines (A), and in HeLa 3D and 5C clones (B). Loading was normalized by cell count. In B, samples were serially diluted 4-fold. Cell lysates were probed in western blots with anti-CypA antibody (upper panel) and anti-β-actin antibody (lower panel).