The effect of CsA on transduction of HeLa cell clones 3D and 5C by HIV-1. WT or CA mutant HIV-1 reporter viruses were used to challenge HeLa 3D (left) or 5C (right) clones treated with 5 μM CsA or DMSO as control. 72 hours later GFP expression was assessed by flow cytometry (A). 24 hours later, late reverse transcription (B) and 2-LTR circles (C) were assayed by quantitative PCR. Data represent one of at least three independent experiments. Error bars represent ± SEM (n = 3).