Figure 8From: Cyclophilin A promotes HIV-1 reverse transcription but its effect on transduction correlates best with its effect on nuclear entry of viral cDNAThe effect of CsA on transduction of HeLa cell clones 3D and 5C by HIV-1. WT or CA mutant HIV-1 reporter viruses were used to challenge HeLa 3D (left) or 5C (right) clones treated with 5 μM CsA or DMSO as control. 72 hours later GFP expression was assessed by flow cytometry (A). 24 hours later, late reverse transcription (B) and 2-LTR circles (C) were assayed by quantitative PCR. Data represent one of at least three independent experiments. Error bars represent ± SEM (n = 3).Back to article page