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Figure 2 | Retrovirology

Figure 2

From: Cyclophilin A promotes HIV-1 reverse transcription but its effect on transduction correlates best with its effect on nuclear entry of viral cDNA

Figure 2

Schematic for strategy to amplify viral cDNA and 2-LTR circles. (A) Schematic for the amplification of the final product of reverse transcription. This PCR identifies HIV-1 cDNA formed after the “second jump” of the reverse transcription process. (B) Schematic for the amplification of 2-LTR circles. A forward primer spanning the junction LTR-LTR was used in order to discriminate 2-LTR circles from products of autointegration. The arrows indicate the primers used in the reaction, and the dashed lines indicate the final PCR products.

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