Effect of hT5Cyp on HIV-1 transduction. HeLa clone 3D (A) and MT4 cells (B), stably expressing the WT or mutated (H436Q) hT5Cyp restriction factor, were treated with CsA or DMSO as control, and challenged with HIV-1-GFP reporter viruses bearing WT, G89V or A92E mutant capsid. Expression of GFP was checked by flow cytometry 72 hrs after challenge with the virus. Infectivity relative to WT virus is represented. Data represent one of at least three independent experiments. Error bars represent ± SEM (n = 3).