Figure 5
Kinetics of anti-HERV-K Env antibodies after HIV-1 infection. A,B) Comparison of the anti-SU (A) and anti-TM (B) response before and after HIV-1 infection in 4 patients. The time between the two time points did not exceed 1 month. A 1:200 sera dilution was used for this assay. C) The evaluation of the kinetics of the response was determined by the subtraction of the OD of the time point after infection by the OD of the time point before infection. A negative result meant a decrease of the response while a positive result meant an increased response. D,E) Total IgG (D) or IgM (E) anti-HERV-K TM and SU were assayed by ELISA on the same plate for 5 time points (1:200 dilution) for the patient OP-1830. The thin black line represents the CD4+ T cell count (cells/mm3) and the hatched line the log10 of the HIV-1 copy number/mm3. Statistical analyses were performed using the Mann & Whitney t-test; *p < 0.05. Results are typical of three independent experiments.