Figure 6

The immune response to the SIV Gag insert was measured by ELISA on plates coated with recombinant p55 SIV Gag protein. (A) Group 3 macaques, which received rubella vectors alone (live vector + 7 weeks), or (B) group 1 macaques, which received DNA prime and rubella vector boost (live vector + 4 weeks), produced high-titer anti-Gag antibodies in six out of six animals, as compared to the positive control from a pool of SIV infected macaques. The rubella vaccine control (CL6A empty in panel A) elicited no antibodies. (C) Anti-Gag antibody titers in three immunized macaques from groups 1 and 3 (four weeks after live vector, solid lines) were compared to a panel of four macaques infected 32 weeks earlier with SIV and a pool of infected macaques (dotted lines). Anti-Gag titers elicited by vector immunization were comparable to the antibodies elicited by SIV infection.