Skip to main content
Figure 3 | Retrovirology

Figure 3

From: Linkages between HIV-1 specificity for CCR5 or CXCR4 and in vitrousage of alternative coreceptors during progressive HIV-1 subtype C infection

Figure 3

Changes in alternative coreceptor usage during the transition from R5 to X4 phenotype in subject 1109. Luciferase reporter viruses pseudotyped with “enrolment” (R5, n = 5), “intermediate” (R5, n = 4) or “final” (X4, n = 6) Envs from subject 1109 [8] were used to infect NP2-CD4 cells expressing CXCR4, CCR5, FPRL1, CCR3 or CCR8, and the levels of HIV-1 entry were determined as described in the Methods. The dotted lines indicate the limit of detection of coreceptor activity, as determined by infections with luciferase reporter virus pseudotyped with the non-functional ΔKS Env [44]. Comparative entry levels for each of the individual Envs are shown in Additional file 1: Table S1. The data shown are means and standard deviations of entry levels in the different cell types, and are representative of 3 independent experiments, each performed in triplicate. The individual circles represent mean entry levels of each of the individual Env clones that were performed in triplicate, from a representative experiment.

Back to article page