RNAi affects HIV-1 replication. Latently infected T cells (J1.1) were treated with TNF-α (10 ng/ml) and enoxacin (50 μM) for 48 hours. (A) shows p24 levels by western blot on enoxacin and TNF-α treatment. (B) shows use of 10-23 DNAzyme in sequence specific knockdown of RLC components PACT, TRBP, Dicer and Ago2. (C) shows the levels of p24 on Dicer, RHA, PACT, TRBP and Ago2 knockdown (DNAzyme)/Overexpression and both. GAPDH was used as a loading control.