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Figure 5 | Retrovirology

Figure 5

From: The PKR activator, PACT, becomes a PKR inhibitor during HIV-1 replication

Figure 5

shRNAs against PACT and ADAR1 decrease HIV-1 protein expression and virion production. HEK 293T cells were co-transfected with 0.2 μg pNL4-3 (lane 1–5), and 2 μg of shRNAs NS (lane 1), 2 μg of shRNA1 PACT (lane 2), 2 μg of shRNA2 PACT (lane 3), 2 μg of shRNA ADAR1 (lane 4) and a combination of shRNA2 PACT and shRNA ADAR1 at 1 μg each (lane 5). 100 μg of cell lysates were resolved by SDS-PAGE and analyzed by immunoblotting using antibodies against P-PKR, PKR, HIV-1 p24, PACT, ADAR1, and actin. NS stands for non-specific, P1 and P2 stand for two different shRNAs against PACT and A stands for shRNA against ADAR1.

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