Figure 2
From: The PKR activator, PACT, becomes a PKR inhibitor during HIV-1 replication
Increased ADAR1-PKR and PACT-PKR interactions during HIV-1 infection. Jurkat cells were mock-infected or infected with HIV-1 pNL4-3. Cell lysates were collected at day 15, which corresponds to the peak of infection for the pNL4-3-infected Jurkat cells. These were immunoprecipitated with anti-PKR or anti-ADAR1. 50 μg of proteins from each lysate (input; lanes 1–2) and the PKR (lanes 3–4) or ADAR (lanes 5–6) immunoprecipitated complexes were run on a 10% SDS-PAGE and blotted using anti-PKR, anti-ADAR1 (against full-length p150 and p110), anti-PACT, anti-HIV-p24 and anti-actin.