Four miRNAs reduce levels of SIV virus production. Primary macaque macrophages were transfected with 100 nM miRNA mimics, then infected with SIV (MOI 0.05) 24 hours after transfection. Supernatants were collected at 24, 48 and 72 hours post-infection and analyzed for virus production. Data shown is an average of at least 3 experiments for each miRNA at each time point. (x) indicates levels at or below the limit of detection, ~60 pg/ml (gray line). Results reported are p27 pg/ml values averaged from at least 3 separate experiments. All statistics reported are the result of a paired, two-sided t test performed on p27 values of time-point matched samples with untransfected controls. * = p < 0.05, ** = p < 0.01, *** = p < 0.001.