B cell lymphoma in hiv transgenic mice

Retrovirology

Table 2 Oncogenes expression in HIV Tg mice

		T/B	BCL2	BCL3	BCL6	C-REL	MUM1	C-MYC	CYC D1	SYK	K-RAS	ABL
M4	S	77(2/58)	0.09	2.51	8.75	0.14	0.02	2.25	14.13	0	0.79	1.95
	L
M9	S	nd	1.00	0.94	1.32	0.39	0.69	0.58	0.81	nd	1.59	nd
	L
M24	S	nd	3.7	2.55	4.9	6.15	0.71	1.99	3.65	2.12	1.98	nd
	L	nd	2.68	1.00	0.42	2.91	0.35	0.04	0.54	0.33	1.63	1.07
M25	S	32(47/54)	4.99	4.98	8.88	4.42	0.75	0.89	2.85	0.60	0.80	0.76
	L	nd	3.13	0.06	1.00	7.14	0.64	0.64	0.26	3.88	3.16	1.48
M1	S	26(31/58)	2.77	1.14	1.58	1.59	1.14	0.23	1.68	0.02	1.44	1.24
	L	71(77/23)	0.9	2.5	1.06	0.78	3.49	0.43	0.59	2.56	0.63	nd
M3	S	25(16/77)	2.52	3.67	2.38	6.62	0.67	3.6	1.88	7.7	2.31	nd
	L	nd
M15	S	23(25/74)	0.28	0.61	1.02	0.12	0.19	0.93	0.70	1.56	2.31	1.59
	L	nd	2.93	0.32	1.80	6.95	0.4	0.77	0.28	1.98	2.83	1.12
M22	S	61(5/69)	0.32	1.39	0.71	0.27	0.95	0.32	0.88	1.34	0.18	5.91
	L	93(70/30)	3.33	0.68	0.19	3.33	0.94	0.52	0.25	0.87	5.33	8.39
M21	S	60(3/60)	1.98	0.96	2.64	1.03	2.56	1.92	3.92	2.46	0.44	1.91
	L	80(30/70)	0.92	0.79	0.19	2.45	1.28	0.07	0.65	3.66	3.93	2.25
M2	S	20(4//86)	0.46	0.20	1.09	0.07	4.21	2.52	8.75	0.06	2.29	2.67
	L	25(25/75)	0.58	0.4	1.12	1.07	1.7	3.23	0.47	3.18	1.5	5.01
M7	S	80(4/96)	0.21	0.53	0.22	0.46	5.1	0.60	0.41	2.32	2.98	2.27
	L	54(27/75)	0.43	0.39	0.41	2.32	5.78	2.56	0.8	8.75	9.39	9.19
M11	S	85(2/90)	1.74	3.27	5.17	8.23	7.02	2.74	1.23	8.69	2.89	3.84
	L	88(1/92)	0.04	2.69	0.2	2.83	5.6	0.78	0.13	4.6	1.89	3.41
M13	S	40(2/77)	0.44	1.57	1.94	2.69	2.99	1.78	0.61	3.45	2.77	0.17
	L	40(5/95)	2.29	0.70	0.24	9.19	4.74	0.30	0.56	21.45	8.96	6.4
M16	S	35(5/83)	3.50	0.80	3.68	13.48	2.07	1.81	5.13	8.4	19.45	nd
	L	53(15/85)	0.73	1.49	0.28	4.52	0.95	3.61	4.91	2.53	16.76	8.23
M20	S	77(2/58)	0.21	0.86	0.23	0.23	0.46	0.68	0.72	1.41	0.25	0.93
	L	75(1/99)	0.06	0.22	0.07	0.89	2.7	0.96	0.15	4.64	2.01	5.31
M28	S	55(2/90)	0.38	0.72	0.69	1.03	5.96	15.38	0.41	1.51	6.28	0.11
	L	nd	0.42	0.21	0.18	0.55	nd	5.54	0.28	1.98	2.1	3.35

Total RNA was isolated from spleen and lymph node of mice at different stage of splenomegaly and was analyzed using primers specific for oncogenes associated to lymphoma (Additional file 1: Table S2) by SYBR Green semiquantitative real-time RT-PCR as described in material and methods. The fold change in each oncogene mRNA compared to the wild type FVBN control is shown relative to the change in the expression of β-actin that was measured as an internal control. The expression of each oncogene in the wild type FVBN control was set to 1. Each mouse is designated with M followed by a number (first column). For each mouse the first line represents the spleen (S), second line represents lymph nodes (L).T/B ratio is indicated in parenthesis and is calculated on total activated cells.

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