Figure 2

HIV proteins expression in HIV Tg mice. (A) gp120 expression in HIV Tg mice. Protein lysates from mice spleen and lymph node were separated by SDS-PAGE and analyzed by western blot using anti-gp120 antibody as described under “Experimental Procedures”. The mice analyzed are indicated on the top of the figure: WT is the mouse control FVB/N, Tg is an HIV Tg mice without skin lesions, mice number 18, 21, and 22 represent mice at the pre-lymphadenopathy stage while mice number 11, 20 and 35 represent mice at a late stage of lymphadenopathy. The numbers in the right represent the protein molecular weight in KDa. (B) Histogram showing the fold changes in gp120 expression in the lymph node versus the spleen from the same mouse. The relative intensity of gp120 was quantified by densitometry and normalized with the beta actin. (C) Western blot for p17 and nef proteins expression in HIV Tg mice using anti-p17 antibody and HIV-1 Nef antiserum as described under “Experimental Procedures”. The mice analyzed are indicated on the top of the figure: WT are mice wild type control FVB/N, Tg are HIV Tg mice without skin lesions, Tg pre-L are mice at pre-lymphadenopathy stage and Tg-L are mice at the last stage of lymphadenopathy. The protein molecular weights are represented in the right of the figure.