Abnormal lymphoid phenotype in HIV Tg mice. (A-B) Skin lesions in HIV-Tg mice. (A) Fungating lesions in the skin; (B) Histology shows a papilloma (low power). C-F Multi-organ involvement by lymphoma in the HIV-Tg mice. (C) Hepatosplenomegaly and lymphadenopathy (gross). (D) Spleen weight/total body weight ratio in HIV Tg mice compared to control wild type FVB/N. 10 HIV Tg and 10 control animals were analyzed. The spleen weight/body weight ratios were approximately 23 times greater than that of wild type (WT) mice (p=0.0004). (E) Portal infiltration by lymphoma cells in the liver (H&E; x400); (F) Lymphomatous infiltration of the lymph node (H&E; x400). G-H Comparison of control and HIV-Tg mouse spleen. (G) Normal splenic architecture in control mice (H&E; x400); (H) Disorganized splenic architecture with atypical lymphoid infiltration and numerous hyperplastic megakaryocytes (H&E; x400). (I) Wright-Giemsa staining of peripheral blood smears. The staining shows circulating lymphoblasts with scant basophilic cytoplasm, high nuclei to cytoplasm ratio, round to irregular nuclei, slightly clumped chromatin, and inconspicuous nucleoli. Small cytoplasmic and nuclear vacuoles were occasionally seen.