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Table 1 HIV-1 integration datasets from in vitro models of latency

From: HIV latency and integration site placement in five cell-based models

Title Cell type Virus Time of harvest after infection Sequencing Generation of expressed vs. silent/inducible Citation Silent/inducible unique sites Expressed unique sites
Jurkat Jurkat cells HIV vector pEV731(LTR-Tat-IRES-GFP) 2 weeks Sanger TNF α, GFP expression [19] 463inducible 643
Bcl-2 transduced CD4 + Primary CD4 +T cells (Bcl-2transduced) HIV NL4-3- Δ6-drEGFP(inactivated gag, vif,vpr, vpu, nef and envreplaced by GFP) 3 days + 3-4weeks + 3 days Sanger Anti-CD3, anti-CD28 antibodies, GFP expression [20] 446inducible 273
Active CD4 + Primary activeCD4 + T cells HIV NL4-3 3 days 454 High vs. low Gag [21] 1604silent 1274
Resting CD4 + Primary restingCD4 + T cells HIV NL4-3 3 days 454 High vs. low Gag [21] 1942silent 784
Central Memory CD4 + Primary centralmemory CD4 +T cells HIV NL4-3 ΔNef GFP 2 days/9 days IonTorrent Anti-CD3, anti-CD28 antibodies, GFP expression This paper 1729inducible 3278