Figure 3

Selection of HIV-1 RT drug-resistance mutations following ARV drug exposure in maternal plasma samples. Amino acid alignment of pol gene regions indicating single point mutations associated with high-level resistance to ARV drugs in subject 4403. CON denotes amino acid consensus sequence from the earliest maternal plasma sample. Bold amino acid residues in the consensus correspond to selected drug-resistant point mutations. Point mutations numbered 1–4 are as follows: 1 for K103N, 2 for V106M, 3 for Y188C/L, 4 for G190A. Dashes denote identity with consensus, while letters correspond to amino acid changes. Asterisks indicate sequences derived from near end-point diluted PCR positive wells (50% positive PCR wells). All other HIV pol sequences were derived by SGA as described [19]. bmL denotes milk sequences derived from left breast; all other sequences are derived from blood plasma. Low-diversity clonally-amplified sequences (0–2 nucleotides away from consensus; bmL_pG5 is a recombinant virus harboring a cluster of 6 nucleotide mismatches shared with more divergent virus sequences) are indicated with brackets.