Figure 6
From: Quantitative analysis of the time-course of viral DNA forms during the HIV-1 life cycle
Efficiency of the 3′-processing reaction during a single round of replication. MT4 cells were infected with the VSV-G-pseudotyped NLENG1-ES-IRES WT (+/- RAL 500 nM) or NLENG1-ES-IRES D116N viruses. (A) WT; (B) WT + 500 nM RAL; (C) D116N: unprocessed linear DNA (uDNAL, white circles); 3′-processed linear DNA (pDNAL, black squares). (D) Percentage of pDNAL (pDNAL over DNAL) during the time course of infection (WT: black columns, WT + 500 nM RAL: grey columns, D116N: white columns). Each value corresponds to an average of five to six independent experiments and confidence intervals analysis are shown for a p value <0.05.