Figure 3
From: Quantitative analysis of the time-course of viral DNA forms during the HIV-1 life cycle
Kinetics of total, circular, linear and integrated DNA forms during a single round of replication. MT4 cells were infected with the VSV-G-pseudotyped NLENG1-ES-IRES WT (+/- RAL 500 nM) or NLENG1-ES-IRES D116N viruses. Each value corresponds to an average of five to six independent experiments and confidence intervals analysis are shown for a p value <0.05. (A) Total viral DNA. (B) Integrated viral DNA. (C) 2-LTRc. (D) Percentage of 2-LTRc (2-LTRc/Total viral DNAx100). (E) 1-LTRc. (F) Percentage of 1-LTRc (1-LTRc/Total viral DNA x 100). (G) Kinetics of total linear viral DNA (uDNAL + pDNAL). WT: black squares; WT + 500 nM RAL: black diamonds; D116N: white circles. (H) Time course of linear viral DNA as a percentage of total viral DNA during infection. WT: black columns; WT + 500nM RAL: grey columns; D116N: white columns.