Figure 4

The 3’ untranslated region of Caspase 8, Aiolos, Ikaros and NPM/B23 mRNAs are targeted by TAR miRNAs. A-D) Schematic of the binding sites for HIV-1 miR-TAR-5p and/or miR-TAR-3p identified in the endogenous 3’UTR of Caspase 8, Aiolos, Ikaros and NPM/B23 mRNAs. Panels show the predicted base pairing and minimal free energy (MFE) calculated for each putative TAR miRNA binding site. E) Schematic of the constructs used for the dual luciferase assays, showing the Renilla luciferase (Rluc) reporter gene coupled to the complete or partial 3’ UTR of each mRNA. F) HEK 293 cells were co-transfected with U6-shNEG (control) or U6-TAR and and the reporter constructs described in panel E, (n = 1 to 3 experiments, in duplicate). Results are expressed as mean ± s.e.m.