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Figure 7 | Retrovirology

Figure 7

From: Identification of novel key amino acids at the interface of the transmembrane domains of human BST-2 and HIV-1 Vpu

Figure 7

Functional validation of the crucial residues in the TMD of Vpu for BST-2 interaction. (a) Western blot analysis of the cellular lysates and the corresponding viral lysates following the cotransfection of the HIV-1 Gag-Pol construct (containing gag and pol genes) and other expressing plasmids into HEK293T cells. (b) Relative BST-2 reduction was determined by densitometric scanning using the ImageJ program (NIH). BST-2 level in cells that were cotransfected with HIV-1 Gag-Pol, R-B and pcDNA was arbitrarily set as 100%. The data shown were the average of three independent experiments. (c) Relative HIV-1 VLPs release was assessed by densitometric scanning using the ImageJ program (NIH). The level of HIV-1 VLPs released from cells that were transfected with HIV-1 Gag-Pol, R-B and V-E was arbitrarily set as 100%. The data shown were the average of three independent experiments.

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Retrovirology

ISSN: 1742-4690

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