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Figure 4 | Retrovirology

Figure 4

From: Identification of novel key amino acids at the interface of the transmembrane domains of human BST-2 and HIV-1 Vpu

Figure 4

The amino acids in TMD of HIV-1 Vpu responsible for human BST-2 interaction. (a) Mutation of the Vm-E TM domain. Every three amino acids were mutated as a group. (b) HEK293T cells were cotransfected with R-B plasmid and a series of Vm-E TM mutants, and the BRET ratio was detected by using a Pekin Elmer reader. Western blot analysis of the cellular lysates was shown. (c) Mutation of the R-B TM domain. (d) HEK293T cells were cotransfected with R-B plasmid and a series of Vm-E TM single amino acid mutants, and the BRET ratio was detected by using a Pekin Elmer reader. Western blot analysis of the cellular lysates was shown.

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Retrovirology

ISSN: 1742-4690

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