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Figure 2 | Retrovirology

Figure 2

From: Identification of novel key amino acids at the interface of the transmembrane domains of human BST-2 and HIV-1 Vpu

Figure 2

The specificity of the BRET assay in detecting the interaction of BST-2 and HIV-1 Vpu. (a) The specificity of BRET signals between BST-2 and Vpu. HEK293T cells were transfected with plasmid DNA and seeded into 96-well plates, and then the BRET ratio was measured by using a Pekin Elmer reader. (b) The saturation curve of the BRET assay. HEK293T cells were cotransfected with constant amount of R-B plasmid and increasing dose of Vm-E or E-CD209 plasmid. The BRET ratio was detected by using a Pekin Elmer reader. Western blot analysis of the cellular lysates was shown. (c) The competition curve of the BRET assay. HEK293T cells were cotransfected with constant amount of R-B plasmid and Vm-E and increasing dose of Vm or CD209 plasmid, and the BRET ratio was detected by using a Pekin Elmer reader. Western blot analysis of the cellular lysates was shown.

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Retrovirology

ISSN: 1742-4690

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