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Figure 3 | Retrovirology

Figure 3

From: HIV-1 capsid undergoes coupled binding and isomerization by the nuclear pore protein NUP358

Figure 3

NUP358Cyp catalyses cis-trans isomerization of HIV-1 CAN. (a-c) 2D 1H-15N ZZ-exchange spectra of HIV-1 CAN focused on Gly89, without enzyme (a), in the presence of NUP358Cyp (b) or CypA (c). Gly89 cis,trans1H,15N correlation or “auto” peaks are labeled and are the result of cis and trans forms of the proceeding Pro90 residue being populated at equilibrium. Addition of either NUP358Cyp or CypA yields exchange peaks that connect cis and trans auto peaks (broken lines). The cis exchange peak appears at the same 1H (15N) chemical shift position as the trans auto peak and vice versa. Note, that the trans exchange peak is largely obscured by additional signals in the spectra. (d-e) The intensities of both auto and exchange peaks vary as a result of the ZZ-mixing time (Tm). Fits of CypA (d) and NUP358Cyp (e) yield exchange constants of 4.3 and 12.1 s-1 respectively.

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