Figure 2
From: HIV-1 capsid undergoes coupled binding and isomerization by the nuclear pore protein NUP358
Co-operative NUP358 residues determine HIV-1 capsid binding. (a) Sequence alignment of NUP358Cyp from primates and other mammals. Residues at position 61 and 113 are highlighted. (b) ITC traces for HIV-1 CAN binding to NUP358Cyp mutants. The location of mutated residues are indicated in the figures below each trace, with residues 61 and 113 shown as space-filling spheres. CypA is indicated in gray and NUP358Cyp in yellow. (c) Structural alignment of CypA (gray) from HIV-1 CAN:CypA complex 1AK4 with HIV-1 CAN:NUP358Cyp (yellow). The CypA-binding loop from HIV-1 CAN is shown in green. Divergent residues in proximity to the binding site are indicated.