Figure 6

Nef induces HIV-1 p55 and p24 localization in membranes of infected primary CD4+T cells. (a) Primary CD4+T cells were infected with VSV-G-pseudotyped WT or ∆Nef. Two days post-infection cells were collected, dounce-homogenized and subjected to membrane flotation analysis as in Figure 5. Representative western blots of WT- (left) or ∆Nef-infected (right) primary CD4+T cells are shown. (b). Quantitative densitometry analysis of the western blots for p55 and p24. The x-axis shows the pixel location in each fraction and y-axis indicates the pixel intensity. (c). The percentages of p55 and p24 found in the membrane fractions was calculated and the mean+SEM of experiments performed with cells from 4 independent donors is shown. *p<0.05 (Mann Whitney test).