Table 1 Determination of sequence accuracy of control samples
Sequence accuracy | Control E6 | Control H8 | |||||
|---|---|---|---|---|---|---|---|
Mean | Minimum | Maximum | Mean | Minimum | Maximum | ||
Overall | % correct nt | 99.89 | 98.37 | 100.00 | 99.90 | 98.96 | 100.00 |
% erroneous nt | 0.11 | 0.00 | 1.63 | 0.10 | 0.00 | 1.04 | |
% mismatches | 0.09 | 0.00 | 1.61 | 0.07 | 0.00 | 0.48 | |
% gaps | 0.02 | 0.00 | 0.94 | 0.03 | 0.00 | 0.70 | |
Non-homopolymeric regions | % correct nt | 99.93 | 99.72 | 100.00 | 99.93 | 99.57 | 100.00 |
% erroneous nt | 0.07 | 0.00 | 0.28 | 0.07 | 0.00 | 0.43 | |
% mismatches | 0.06 | 0.00 | 0.27 | 0.05 | 0.00 | 0.38 | |
% gaps | 0.01 | 0.00 | 0.07 | 0.02 | 0.00 | 0.39 | |
Homopolymeric regions a | % correct nt | 99.67 | 98.37 | 99.99 | 99.82 | 98.96 | 100.00 |
% erroneous nt | 0.33 | 0.01 | 1.63 | 0.18 | 0.00 | 1.04 | |
% mismatches | 0.25 | 0.01 | 1.61 | 0.11 | 0.00 | 0.48 | |
% gaps | 0.08 | 0.00 | 0.94 | 0.07 | 0.00 | 0.70 | |